Hotspot

trnG-GCC-trnM-CAU

LSC · 40702-40891 · 190 bp

Back to Gymnocladus

Pi 0.0703
MarkerSeek score 82.50
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
190
variable_sites
13
pi
0.0703
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 AAGGACGATCAAAATGCGGA CTTGAGGTCACGGGTTCAAA 45.0 50.0 57.9 58.0 310-334 bp 1.0 83.3
trnG-GCC-trnM-CAU_p2 CAAGGACGATCAAAATGCGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 311-335 bp 1.0 82.7
trnG-GCC-trnM-CAU_p3 AAGGACGATCAAAATGCGGA TGAGGTCACGGGTTCAAATC 45.0 50.0 57.9 57.8 308-332 bp 1.0 82.2
trnG-GCC-trnM-CAU_p4 AAGGACGATCAAAATGCGGA GTAGCTCGCAAGGCTCATAA 45.0 50.0 57.9 57.8 331-355 bp 1.0 81.5
trnG-GCC-trnM-CAU_p5 AAGGACGATCAAAATGCGGA TAGCTCGCAAGGCTCATAAC 45.0 50.0 57.9 57.8 330-354 bp 1.0 81.5