Hotspot

trnR-UCU-atpA

LSC · 11975-12322 · 348 bp

Back to Gymnocladus

Pi 0.0818

MarkerSeek score 78.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 27
pi: 0.0818
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CATTCACTCGGGAAGCAGAA	47.6	50.0	57.3	57.9	462-556 bp	1.0	72.7
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	47.6	50.0	57.3	57.9	466-560 bp	1.0	72.7
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	452-546 bp	1.0	70.6
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ATTCACTCGGGAAGCAGAAG	47.6	50.0	57.3	57.6	461-555 bp	1.0	70.4
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	CCAAGACATTCACTCGGGAA	47.6	50.0	57.3	57.5	468-562 bp	1.0	69.7