Hotspot

petD

LSC · 83124-84344 · 1221 bp

Back to Gymnema

Pi 0.00661

MarkerSeek score 51.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1221
variable_sites: 12
pi: 0.00661
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TGTTGAGGGGAATTCTCTGA	TCCCTAGATACACACGTCGT	45.0	50.0	55.8	57.6	1282-1292 bp	1.0	60.2
petD_p2	TGTTGAGGGGAATTCTCTGAA	TCCCTAGATACACACGTCGT	42.9	50.0	56.6	57.6	1282-1292 bp	1.0	58.6
petD_p3	TGTTGAGGGGAATTCTCTGAAT	TCCCTAGATACACACGTCGT	40.9	50.0	57.1	57.6	1282-1292 bp	1.0	54.7
petD_p4	TTGTTGAGGGGAATTCTCTGA	TCCCTAGATACACACGTCGT	42.9	50.0	56.6	57.6	1293 bp	0.333333	30.5
petD_p5	TTGTTGAGGGGAATTCTCTGAA	TCCCTAGATACACACGTCGT	40.9	50.0	57.3	57.6	1293 bp	0.333333	28.3