Hotspot

ycf2-trnL-CAA

IRb · 100125-100687 · 563 bp

Back to Gymnema

Pi 0.00474

MarkerSeek score 61.20

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 563
variable_sites: 4
pi: 0.00474
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	ATGGCTTTTCCCGGATGAAA	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	671-672 bp	1.0	78.7
ycf2-trnL-CAA_p2	TGGCTTTTCCCGGATGAAAT	AAATGGTAGACACGCGAGAC	45.0	50.0	57.8	58.0	670-671 bp	1.0	78.7
ycf2-trnL-CAA_p3	ATGGCTTTTCCCGGATGAAA	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	673-674 bp	1.0	78.7
ycf2-trnL-CAA_p4	TGGCTTTTCCCGGATGAAAT	TGAAATGGTAGACACGCGAG	45.0	50.0	57.8	58.0	672-673 bp	1.0	78.7
ycf2-trnL-CAA_p5	AAGATGGCTTTTCCCGGATG	AAATGGTAGACACGCGAGAC	50.0	50.0	58.2	58.0	674-675 bp	1.0	78.7