Hotspot

petB-petD

LSC · 82936-83123 · 188 bp

Back to Gymnema

Pi 0.0471

MarkerSeek score 63.20

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 13
pi: 0.0471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	CAATCCACCTTGACTCGGTT	TTCCAATCCCAATGTGGCAA	50.0	45.0	57.8	58.0	376 bp	1.0	82.0
petB-petD_p2	CAATCCACCTTGACTCGGTT	ATTCCAATCCCAATGTGGCA	50.0	45.0	57.8	57.7	377 bp	1.0	80.1
petB-petD_p3	CAATCCACCTTGACTCGGTT	GTTCAAGTCACACACTCCCA	50.0	50.0	57.8	57.7	329 bp	1.0	80.0
petB-petD_p4	CAATCCACCTTGACTCGGTT	AATCCCAATGTGGCAAGCAT	50.0	45.0	57.8	58.4	372 bp	1.0	78.8
petB-petD_p5	CAATCCACCTTGACTCGGTT	TCCAATCCCAATGTGGCAAG	50.0	50.0	57.8	58.4	375 bp	1.0	78.6