Hotspot

psaA-ycf3

LSC · 47463-48335 · 873 bp

Back to Gymnema

Pi 0.0115

MarkerSeek score 66.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 873
variable_sites: 15
pi: 0.0115
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCTGCCGAGTATTGG	50.0	50.0	58.1	58.0	1070-1111 bp	1.0	80.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCTGCCGAGTATTGG	50.0	50.0	57.9	58.0	1051-1092 bp	1.0	79.9
psaA-ycf3_p3	TGAGAAATGACCCGGTTTGG	CGATCAAGCTGCCGAGTATT	50.0	50.0	58.1	58.1	1072-1113 bp	1.0	79.3
psaA-ycf3_p4	TGAGAAATGACCCGGTTTGG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	999-1040 bp	1.0	79.2
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CGATCAAGCTGCCGAGTATT	50.0	50.0	57.9	58.1	1053-1094 bp	1.0	79.1