Hotspot

trnE-UUC-trnT-GGU

LSC · 36246-37124 · 879 bp

Back to Gymnema

Pi 0.00389
MarkerSeek score 65.00
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
879
variable_sites
5
pi
0.00389
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 966-991 bp 1.0 82.0
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 964-989 bp 1.0 82.0
trnE-UUC-trnT-GGU_p3 GATGTCCTGAACCGCTAGAC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 963-988 bp 1.0 81.5
trnE-UUC-trnT-GGU_p4 CCTTTCCTAGTCCCCTACCC GAACCGATGACTTACGCCTT 60.0 50.0 58.2 58.0 1022-1047 bp 1.0 81.4
trnE-UUC-trnT-GGU_p5 CTTTCCTAGTCCCCTACCCC GAACCGATGACTTACGCCTT 60.0 50.0 58.2 58.0 1021-1046 bp 1.0 81.4