Hotspot

trnC-GCA-petN

LSC · 32883-33598 · 716 bp

Back to Gymnema

Pi 0.00559
MarkerSeek score 62.30
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
716
variable_sites
6
pi
0.00559
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 AGAAAGTTCCGTACTGCGTC ACTTCTTCCCCACACTACGA 50.0 50.0 57.9 58.0 907-1083 bp 1.0 81.0
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG ACTTCTTCCCCACACTACGA 50.0 50.0 58.1 58.0 826-1002 bp 1.0 80.9
trnC-GCA-petN_p3 AGAAAGTTCCGTACTGCGTC CACTACGAGCGAAAGGGAAA 50.0 50.0 57.9 57.9 895-1071 bp 1.0 80.4
trnC-GCA-petN_p4 AGAAAGTTCCGTACTGCGTC CACTTCTTCCCCACACTACG 50.0 55.0 57.9 57.9 908-1084 bp 1.0 80.4
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG CACTACGAGCGAAAGGGAAA 50.0 50.0 58.1 57.9 814-990 bp 1.0 80.4