Hotspot

rps2-rpoC2

LSC · 16688-21622 · 4935 bp

Back to Gymnema

Pi 0.00565

MarkerSeek score 48.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 4935
variable_sites: 37
pi: 0.00565
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CTTAGCATACCAAGGGGCTG	CTCCGGGGGCTATTAGAGAA	55.0	55.0	58.0	58.0	1060-1167 bp	1.0	84.8
rps2-rpoC2_p2	CTTAGCATACCAAGGGGCTG	TCCGGGGGCTATTAGAGAAG	55.0	55.0	58.0	58.0	1059-1166 bp	1.0	84.8
rps2-rpoC2_p3	CTTAGCATACCAAGGGGCTG	AACTTGAAGCCCCTCTCTCT	55.0	50.0	58.0	58.0	1127-1234 bp	1.0	84.6
rps2-rpoC2_p4	CTTAGCATACCAAGGGGCTG	ACTTGAAGCCCCTCTCTCTT	55.0	50.0	58.0	58.0	1126-1233 bp	1.0	84.6
rps2-rpoC2_p5	CTTAGCATACCAAGGGGCTG	CGGCATTCTGTTCTACAGCT	55.0	50.0	58.0	58.0	603-700 bp	0.666667	70.9