Hotspot

trnS-GCU-trnG-UCC

LSC · 8710-9303 · 594 bp

Back to Gymnema

Pi 0.0185

MarkerSeek score 64.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 594
variable_sites: 16
pi: 0.0185
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	TCAAACCGAAAACAAAGACCC	45.0	42.9	58.0	57.2	713-790 bp	1.0	67.4
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	CAAACCGAAAACAAAGACCCT	45.0	42.9	58.0	57.2	712-789 bp	1.0	67.3
trnS-GCU-trnG-UCC_p3	CATCAAGGGAAGCGGAAAGA	TCAAACCGAAAACAAAGACCC	50.0	42.9	57.9	57.2	773-850 bp	1.0	66.6
trnS-GCU-trnG-UCC_p4	TCATCAAGGGAAGCGGAAAG	TCAAACCGAAAACAAAGACCC	50.0	42.9	57.9	57.2	774-851 bp	1.0	66.6
trnS-GCU-trnG-UCC_p5	CATCAAGGGAAGCGGAAAGA	CAAACCGAAAACAAAGACCCT	50.0	42.9	57.9	57.2	772-849 bp	1.0	66.6