Hotspot

petD

LSC · 75464-76753 · 1290 bp

Back to Gymnadenia

Pi 0.00171

MarkerSeek score 38.60

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1290
variable_sites: 5
pi: 0.00171
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	CAGTTCATCGATGGTCAGCA	ATCCCCTTCTGGATCTTCCC	50.0	55.0	58.0	58.2	1936-1938 bp	0.8	70.6
petD_p2	AACCGTCACAGAGGCTTTTT	ATCCCCTTCTGGATCTTCCC	45.0	55.0	57.9	58.2	2007-2009 bp	0.8	70.3
petD_p3	TGTGTATCTTACGGGTGGGT	ATCCCCTTCTGGATCTTCCC	50.0	55.0	58.1	58.2	1878-1880 bp	0.8	70.2
petD_p4	CGTGTGTATCTTACGGGTGG	ATCCCCTTCTGGATCTTCCC	55.0	55.0	58.1	58.2	1880-1882 bp	0.8	70.0
petD_p5	CTATCGTCCAACCGTCACAG	ATCCCCTTCTGGATCTTCCC	55.0	55.0	58.1	58.2	2016-2018 bp	0.8	70.0