Hotspot

petB

LSC · 73893-75278 · 1386 bp

Back to Gymnadenia

Pi 0.00231

MarkerSeek score 53.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1386
variable_sites: 7
pi: 0.00231
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CCATTCTGGTAGTTCGATCGT	ACTCTTGTTCCTCCCCGATA	47.6	50.0	57.6	57.5	1490-1497 bp	1.0	64.8
petB_p2	CCATTCTGGTAGTTCGATCGT	GACTCTTGTTCCTCCCCGAT	47.6	55.0	57.6	58.8	1491-1498 bp	1.0	62.7
petB_p3	CCATTCTGGTAGTTCGATCGT	AAGACTCTTGTTCCTCCCCG	47.6	55.0	57.6	59.0	1493-1500 bp	1.0	61.0
petB_p4	CCATTCTGGTAGTTCGATCGT	TCTTGTTCCTCCCCGATATGA	47.6	47.6	57.6	58.0	1488-1495 bp	1.0	60.8
petB_p5	CCATTCTGGTAGTTCGATCGT	ACTCTTGTTCCTCCCCGATAT	47.6	47.6	57.6	58.0	1490-1497 bp	1.0	60.8