Hotspot

atpF

LSC · 11149-12562 · 1414 bp

Back to Gymnadenia

Pi 0.00424

MarkerSeek score 44.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1414
variable_sites: 14
pi: 0.00424
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACCTAAACTCTAAACTGTAAACT	GGCGAGCGGAGTGATAAAAA	30.4	50.0	53.0	58.6	1532-1534 bp	1.0	40.9
atpF_p2	ACTAGAGTAAATAGTCAAAAAGCT	GGCGAGCGGAGTGATAAAAA	29.2	50.0	53.7	58.6	1512-1514 bp	1.0	40.9
atpF_p3	ACCTAAACTCTAAACTGTAAACT	GGCGAGCGGAGTGATAAAA	30.4	52.6	53.0	57.9	1532-1534 bp	1.0	40.9
atpF_p4	ACCTAAACTCTAAACTGTAAACTAGA	GGCGAGCGGAGTGATAAAAA	30.8	50.0	55.6	58.6	1532-1534 bp	1.0	40.9
atpF_p5	ACCTAAACTCTAAACTGTAAACTAGAG	GGCGAGCGGAGTGATAAAAA	33.3	50.0	56.6	58.6	1532-1534 bp	1.0	40.9