Hotspot

trnP-UGG-psaJ

LSC · 64753-65119 · 367 bp

Back to Gymnadenia

Pi 0.0153

MarkerSeek score 52.40

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 367
variable_sites: 11
pi: 0.0153
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	497-523 bp	1.0	80.5
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	496-522 bp	1.0	80.5
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	508-534 bp	1.0	80.4
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	507-533 bp	1.0	80.4
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TAGACCCGCTAAAGACCCAA	50.0	50.0	58.1	58.1	510-536 bp	1.0	80.4