Hotspot

rbcL-accD

LSC · 55420-56151 · 732 bp

Back to Gymnadenia

Pi 0.00847
MarkerSeek score 42.90
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
732
variable_sites
13
pi
0.00847
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 ACTACAGTTCGGTGGAGGAA CCACTGGTATGTCCAAGAGC 50.0 55.0 58.0 58.0 1082-1088 bp 1.0 81.0
rbcL-accD_p2 ACTACAGTTCGGTGGAGGAA GTCCAAGAGCATCAGCATCA 50.0 50.0 58.0 58.0 1072-1078 bp 1.0 81.0
rbcL-accD_p3 ACTACAGTTCGGTGGAGGAA TGTCCAAGAGCATCAGCATC 50.0 50.0 58.0 58.0 1073-1079 bp 1.0 80.9
rbcL-accD_p4 GGCTTCAGGGGGTATTCATG CCACTGGTATGTCCAAGAGC 55.0 55.0 58.0 58.0 1148-1154 bp 1.0 80.9
rbcL-accD_p5 GGCTTCAGGGGGTATTCATG GTCCAAGAGCATCAGCATCA 55.0 50.0 58.0 58.0 1138-1144 bp 1.0 80.8