Hotspot

ndhF

SSC · 154333-156546 · 2214 bp

Back to Guzmania

Pi 0.286

MarkerSeek score 67.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 4
pi: 0.286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.01
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGCCCAGTTCACGAAGATTC	TGGTGAATCGAGAACAATTTGT	50.0	36.4	57.9	56.6	2365 bp	0.5	31.6
ndhF_p2	GCCCAGTTCACGAAGATTCT	TGGTGAATCGAGAACAATTTGT	50.0	36.4	57.9	56.6	2364 bp	0.5	31.6
ndhF_p3	AGCCCAGTTCACGAAGATTC	AGAATTGGTGAATCGAGAACA	50.0	38.1	57.9	55.2	2370 bp	0.5	29.1
ndhF_p4	GCCCAGTTCACGAAGATTCT	AGAATTGGTGAATCGAGAACA	50.0	38.1	57.9	55.2	2369 bp	0.5	29.1
ndhF_p5	GCCCAGTTCACGAAGATTCT	TTGGTGAATCGAGAACAATTTGT	50.0	34.8	57.9	57.2	2365 bp	0.5	29.1