Hotspot

ndhA

SSC · 6667-8811 · 2145 bp

Back to Guzmania

Pi 0.00886

MarkerSeek score 52.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2145
variable_sites: 19
pi: 0.00886
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CGTTTCTTTCTCTTGTTTGATAGAGA	TGGGAGAAGTTGATCGTTGA	34.6	45.0	57.7	56.5	2228 bp	1.0	40.7
ndhA_p2	CGTTTCTTTCTCTTGTTTGAT	TGGGAGAAGTTGATCGTTGA	33.3	45.0	52.7	56.5	2228 bp	1.0	40.7
ndhA_p3	CGTTTCTTTCTCTTGTTTGATAGAG	TGGGAGAAGTTGATCGTTGA	36.0	45.0	56.5	56.5	2228 bp	1.0	40.7
ndhA_p4	CGTTTCTTTCTCTTGTTTGATAGAGA	TGGGAGAAGTTGATCGTTGAA	34.6	42.9	57.7	57.2	2228 bp	1.0	40.7
ndhA_p5	CGTTTCTTTCTCTTGTTTGAT	TGGGAGAAGTTGATCGTTGAA	33.3	42.9	52.7	57.2	2228 bp	1.0	40.7