Hotspot

rps11-rpl36

LSC · 123160-123204 · 45 bp

Back to Guzmania

Pi 0.0444

MarkerSeek score 78.10

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 45
variable_sites: 2
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	GAACCAATACGCCCATTCCT	GCAGGCGTGGACGAATTATA	50.0	50.0	57.9	58.1	160-260 bp	1.0	79.2
rps11-rpl36_p2	GAACCAATACGCCCATTCCT	AAGGGCTTCCGTTCGTAAAA	50.0	45.0	57.9	57.7	204-304 bp	1.0	78.1
rps11-rpl36_p3	AATACGCCCATTCCTACGTG	GCAGGCGTGGACGAATTATA	50.0	50.0	57.8	58.1	155-255 bp	1.0	77.8
rps11-rpl36_p4	CAATACGCCCATTCCTACGT	GCAGGCGTGGACGAATTATA	50.0	50.0	57.8	58.1	156-256 bp	1.0	77.8
rps11-rpl36_p5	TGAACCAATACGCCCATTCC	GCAGGCGTGGACGAATTATA	50.0	50.0	58.2	58.1	161-261 bp	1.0	77.7