Hotspot

psbE-petL

LSC · 107927-109429 · 1503 bp

Back to Guzmania

Pi 0.00695

MarkerSeek score 66.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1503
variable_sites: 10
pi: 0.00695
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1604-1650 bp	1.0	78.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1603-1649 bp	1.0	78.0
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1617-1663 bp	1.0	77.6
psbE-petL_p4	ACTGACGAATAACCAACCCG	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1616-1662 bp	1.0	77.6
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCAGCCAGTAGAAAACCAA	45.0	45.0	58.2	57.3	1612-1658 bp	1.0	77.0