Hotspot

petA-psbJ

LSC · 106020-107157 · 1138 bp

Back to Guzmania

Pi 0.0197

MarkerSeek score 59.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 22
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	50.0	50.0	57.9	58.0	1241-1264 bp	1.0	82.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	50.0	50.0	57.9	58.0	1239-1262 bp	1.0	82.1
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	50.0	55.0	57.9	57.6	1240-1263 bp	1.0	79.2
petA-psbJ_p4	GCCTTTTGTTCTTCTTCGCA	TCTGGGTTGGGTTCATCTCT	45.0	50.0	57.2	58.0	1240-1263 bp	1.0	76.3
petA-psbJ_p5	GCCTTTTGTTCTTCTTCGCA	TGGGTTGGGTTCATCTCTCT	45.0	50.0	57.2	58.0	1238-1261 bp	1.0	76.3