Hotspot

ycf4-cemA

LSC · 103281-104136 · 856 bp

Back to Guzmania

Pi 0.00719

MarkerSeek score 65.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 856
variable_sites: 6
pi: 0.00719
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCCGAATTGG	TGGGAAAGAAGGCAATGCTT	45.0	45.0	57.8	58.0	928-944 bp	1.0	81.0
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.8	57.8	974-990 bp	1.0	79.7
ycf4-cemA_p3	GCCATTCCCTTGACTCGTAC	TGGGAAAGAAGGCAATGCTT	55.0	45.0	58.3	58.0	978-994 bp	1.0	79.3
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	ATGGGAAAGAAGGCAATGCT	45.0	45.0	57.8	57.8	929-945 bp	1.0	79.1
ycf4-cemA_p5	GCCATTCCCTTGACTCGTAC	ATGAGAGAGAGACCCACCAG	55.0	55.0	58.3	57.8	1024-1040 bp	1.0	78.1