Hotspot

accD-psaI

LSC · 101735-102274 · 540 bp

Back to Guzmania

Pi 0.0167

MarkerSeek score 58.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 540
variable_sites: 9
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	669-1046 bp	1.0	78.8
accD-psaI_p2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	668-1045 bp	1.0	78.8
accD-psaI_p3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	675-1052 bp	1.0	78.2
accD-psaI_p4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	674-1051 bp	1.0	78.2
accD-psaI_p5	GCTCCATGGTTTCTTTCCCT	CCGGAAATACTAGGCCCACT	50.0	55.0	57.8	58.9	656-1033 bp	1.0	72.1