Hotspot

ndhC-trnV-UAC

LSC · 93277-94322 · 1046 bp

Back to Guzmania

Pi 0.0205
MarkerSeek score 72.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1046
variable_sites
21
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1183-1212 bp 1.0 81.0
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAACTCC GAGCCCAATGCAACAAACAA 55.0 45.0 58.0 57.8 1270-1299 bp 1.0 80.5
ndhC-trnV-UAC_p3 CGGGGCTAAAACTCCTGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1178-1207 bp 1.0 79.8
ndhC-trnV-UAC_p4 CGGGGCTAAAACTCCTGAAA GAGCCCAATGCAACAAACAA 50.0 45.0 57.8 57.8 1265-1294 bp 1.0 79.2
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAACTCCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1182-1211 bp 1.0 78.6