Hotspot

trnF-GAA-ndhJ

LSC · 91053-91600 · 548 bp

Back to Guzmania

Pi 0.0112

MarkerSeek score 66.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 548
variable_sites: 6
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	645-658 bp	1.0	80.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	635-648 bp	1.0	80.3
trnF-GAA-ndhJ_p3	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	688-701 bp	1.0	79.5
trnF-GAA-ndhJ_p4	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	687-700 bp	1.0	79.5
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.2	58.2	677-690 bp	1.0	79.0