Hotspot

trnG-GCC-trnfM-CAU

LSC · 79317-79449 · 133 bp

Back to Guzmania

Pi 0.0677
MarkerSeek score 83.50
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
133
variable_sites
9
pi
0.0677
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 AGAAGATGCGGGTTCGATTC CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 199 bp 1.0 82.8
trnG-GCC-trnfM-CAU_p2 GAGAAGATGCGGGTTCGATT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 200 bp 1.0 82.8
trnG-GCC-trnfM-CAU_p3 ATGCGGATATGGTCGAATGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.9 58.0 240 bp 1.0 81.9
trnG-GCC-trnfM-CAU_p4 GTGTGGACATATACGTGCGT CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 276 bp 1.0 81.6
trnG-GCC-trnfM-CAU_p5 TGTGTGGACATATACGTGCG CTTGAGGTCACGGGTTCAAA 50.0 50.0 58.1 58.0 277 bp 1.0 81.6