Hotspot

rps16-trnQ-UUG

LSC · 47820-48190 · 371 bp

Back to Guzmania

Pi 0.0216

MarkerSeek score 66.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 371
variable_sites: 8
pi: 0.0216
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAAAATCCACAGCCGACCAT	GAAATCAAAATGGGGCGTGG	45.0	50.0	58.1	58.0	529 bp	1.0	80.5
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAAATCAAAATGGGGCGTGG	45.0	50.0	58.1	58.0	502 bp	1.0	80.1
rps16-trnQ-UUG_p3	AAAAATCCACAGCCGACCAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	471 bp	1.0	80.1
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	444 bp	1.0	79.7
rps16-trnQ-UUG_p5	CCGACCATGTCCTTCAAGTC	GAAATCAAAATGGGGCGTGG	55.0	50.0	58.3	58.0	517 bp	1.0	79.0