Hotspot

rps15-ycf1

SSC · 10366-10774 · 409 bp

Back to Guzmania

Pi 0.0163

MarkerSeek score 53.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 409
variable_sites: 6
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	TCCTAGACGTCGCAGAGAAT	45.0	50.0	58.1	58.0	1114-1162 bp	1.0	83.5
rps15-ycf1_p2	AATAAGCCAGCAACCGTTGA	TCCTAGACGTCGCAGAGAAT	45.0	50.0	58.1	58.0	876-915 bp	1.0	83.5
rps15-ycf1_p3	CCAATTGTTCTGGCGCATTT	TCCTAGACGTCGCAGAGAAT	45.0	50.0	57.9	58.0	1307-1355 bp	1.0	82.7
rps15-ycf1_p4	TTCTGGCGCATTTACGGTTA	TCCTAGACGTCGCAGAGAAT	45.0	50.0	57.9	58.0	1300-1348 bp	1.0	82.6
rps15-ycf1_p5	AACACCATGCATTGATGGGT	GGATGGCTCCTTGAACCAAA	45.0	50.0	58.1	58.1	1522-1570 bp	1.0	82.3