Pi
0.251
MarkerSeek score
80.70
Primer available
yes
Label
ycf89
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf89_p1 | ATTAATGATGAGGCGGCAGG | CATTGCGGAAAATTCCCCAC | 50.0 | 50.0 | 58.1 | 58.0 | 2184 bp | 0.5 | 59.1 |
| ycf89_p2 | ATTAATGATGAGGCGGCAGG | ATATGGGGTTTTAGCAGCCG | 50.0 | 50.0 | 58.1 | 58.0 | 2002 bp | 0.5 | 59.1 |
| ycf89_p3 | ATTAATGATGAGGCGGCAGG | TATTGCTCCGTCAGGCTTTC | 50.0 | 50.0 | 58.1 | 58.0 | 2207 bp | 0.5 | 59.0 |
| ycf89_p4 | ATTAATGATGAGGCGGCAGG | TTTAGCAGCCGTTTCCAACT | 50.0 | 45.0 | 58.1 | 58.0 | 1993 bp | 0.5 | 59.0 |
| ycf89_p5 | ATTAATGATGAGGCGGCAGG | TCGCCTTGGTAAGCCATTAC | 50.0 | 50.0 | 58.1 | 58.0 | 2087 bp | 0.5 | 59.0 |
| ycf89_p1 | CATTGCGGAAAATTCCCCAC | ATTAATGATGAGGCGGCAGG | 50.0 | 50.0 | 58.0 | 58.1 | 2184 bp | 0.5 | 59.1 |
| ycf89_p2 | ATATGGGGTTTTAGCAGCCG | ATTAATGATGAGGCGGCAGG | 50.0 | 50.0 | 58.0 | 58.1 | 2002 bp | 0.5 | 59.1 |
| ycf89_p3 | TATTGCTCCGTCAGGCTTTC | ATTAATGATGAGGCGGCAGG | 50.0 | 50.0 | 58.0 | 58.1 | 2207 bp | 0.5 | 59.0 |
| ycf89_p4 | TTTAGCAGCCGTTTCCAACT | ATTAATGATGAGGCGGCAGG | 45.0 | 50.0 | 58.0 | 58.1 | 1993 bp | 0.5 | 59.0 |
| ycf89_p5 | TCGCCTTGGTAAGCCATTAC | ATTAATGATGAGGCGGCAGG | 50.0 | 50.0 | 58.0 | 58.1 | 2087 bp | 0.5 | 59.0 |