Hotspot

ycf45

IRb · 45645-47024 · 1380 bp

Back to Guinardia

Pi 0.48

MarkerSeek score 81.30

Primer available yes

Label ycf45

Feature Metadata

label_name: ycf45
feature_type: gene
region: IRb
length_bp: 1380
variable_sites: 575
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf45_p1	GGTCAGAGTTAAACGCGGAA	CCTGCCGCCTCATCATTAAT	50.0	50.0	58.2	58.1	2211 bp	0.5	57.5
ycf45_p2	GGTCAGAGTTAAACGCGGAA	AGGTGTAGATGGACGAAGCT	50.0	50.0	58.2	58.1	2003 bp	0.5	57.1
ycf45_p3	ATGGTCAGAGTTAAACGCGG	CCTGCCGCCTCATCATTAAT	50.0	50.0	58.3	58.1	2213 bp	0.5	57.0
ycf45_p4	GGTCAGAGTTAAACGCGGAA	GGTGTAACTTGGTCTGCCTC	50.0	55.0	58.2	58.2	2103 bp	0.5	56.8
ycf45_p5	TGCGGCGTCAAAACTAAATG	CCTGCCGCCTCATCATTAAT	45.0	50.0	57.7	58.1	2016 bp	0.5	56.6