Hotspot

rns

IRb · 49174-50657 · 1484 bp

Back to Guinardia

Pi 0.515

MarkerSeek score 80.10

Primer available yes

Label rns

Feature Metadata

label_name: rns
feature_type: rRNA
region: IRb
length_bp: 1484
variable_sites: 630
pi: 0.515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns_p1	AAATCTCCCTGCTCTCTGGT	CGTCCTTCATCGCTTCTGAA	50.0	50.0	58.0	58.0	2276 bp	0.5	59.7
rns_p2	AAATCTCCCTGCTCTCTGGT	TACTTGTGGAGGTAAGCGGA	50.0	50.0	58.0	58.1	2167 bp	0.5	59.1
rns_p3	AAATCTCCCTGCTCTCTGGT	TCGCAGATTCACACGGAATT	50.0	45.0	58.0	57.9	2646 bp	0.5	59.0
rns_p4	AAATCTCCCTGCTCTCTGGT	AAAAGTTTAGGCTGGTCCCG	50.0	50.0	58.0	58.1	2485 bp	0.5	59.0
rns_p5	AAATCTCCCTGCTCTCTGGT	TATGATTGTCCCGCTACCCT	50.0	50.0	58.0	57.9	2508 bp	0.5	58.9