Hotspot

bas1

IRb · 44326-44937 · 612 bp

Back to Guinardia

Pi 0.486

MarkerSeek score 78.00

Primer available yes

Label bas1

Feature Metadata

label_name: bas1
feature_type: gene
region: IRb
length_bp: 612
variable_sites: 222
pi: 0.486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
bas1_p1	AGAAGCTCCTGCAAAACCTG	ACGTTTCCGCGTTTAACTCT	50.0	45.0	58.4	58.1	976 bp	0.5	55.8
bas1_p2	AGAAGCTCCTGCAAAACCTG	AGACGAGAATGTGCTCCTCT	50.0	50.0	58.4	58.2	1042 bp	0.5	55.7
bas1_p3	AGAAGCTCCTGCAAAACCTG	GACGTTTCCGCGTTTAACTC	50.0	50.0	58.4	57.8	977 bp	0.5	55.3
bas1_p4	AGAAGCTCCTGCAAAACCTG	CGTTTCCGCGTTTAACTCTG	50.0	50.0	58.4	57.8	975 bp	0.5	55.3
bas1_p5	AGAAGCTCCTGCAAAACCTG	TTCCGCGTTTAACTCTGACC	50.0	50.0	58.4	58.2	972 bp	0.5	55.2