Hotspot

acpP

IRb · 47175-47411 · 237 bp

Back to Guinardia

Pi 0.48

MarkerSeek score 71.40

Primer available yes

Label acpP

Feature Metadata

label_name: acpP
feature_type: gene
region: IRb
length_bp: 237
variable_sites: 84
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
acpP_p1	AAGAAGCAAAACGACGAGGT	TGTCACTTCAAAAGCAGCCA	45.0	45.0	58.1	58.2	1229 bp	0.5	57.7
acpP_p2	AAGAAGCAAAACGACGAGGT	CGTTACTCCCAGTTGCTGAG	45.0	55.0	58.1	58.3	1716 bp	0.5	57.3
acpP_p3	AAGAAGCAAAACGACGAGGT	ATAGCGTCCTGGACCGTTAT	45.0	50.0	58.1	58.3	1288 bp	0.5	57.2
acpP_p4	AAGAAGCAAAACGACGAGGT	AGGTAACCGTTACTCCCAGT	45.0	50.0	58.1	57.7	1723 bp	0.5	57.1
acpP_p5	AGCTTCGTCCATCTACACCT	TGTCACTTCAAAAGCAGCCA	50.0	45.0	58.1	58.2	529 bp	0.5	56.9