Hotspot

rnl-trnA(tgc)

IRa · 99507-99569 · 63 bp

Back to Guinardia

Pi 0.164

MarkerSeek score 85.30

Primer available yes

Label rnl-trnA(tgc)

Feature Metadata

label_name: rnl-trnA(tgc)
feature_type: igs
region: IRa
length_bp: 63
variable_sites: 10
pi: 0.164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rnl-trnA(tgc)_p1	CGTCCTTCATCGCTTCTGAA	GCGAGGTCTCTGGTTCAAAT	50.0	50.0	58.0	57.9	221-223 bp	1.0	83.5
rnl-trnA(tgc)_p2	TCATCGCTTCTGAATGCCAA	GCGAGGTCTCTGGTTCAAAT	45.0	50.0	57.9	57.9	215-217 bp	1.0	82.7
rnl-trnA(tgc)_p3	TTCATCGCTTCTGAATGCCA	GCGAGGTCTCTGGTTCAAAT	45.0	50.0	57.9	57.9	216-218 bp	1.0	82.7
rnl-trnA(tgc)_p4	GTATCGTCGGTAACCACGTC	GCGAGGTCTCTGGTTCAAAT	55.0	50.0	58.2	57.9	237-239 bp	1.0	81.7
rnl-trnA(tgc)_p5	CGTCCTTCATCGCTTCTGAA	CTGATAAGGGCGAGGTCTCT	50.0	55.0	58.0	58.3	230-232 bp	1.0	81.7