Hotspot

rrn5-psbA

IRb · 53986-54470 · 485 bp

Back to Guinardia

Pi 0.429

MarkerSeek score 79.40

Primer available yes

Label rrn5-psbA

Feature Metadata

label_name: rrn5-psbA
feature_type: igs
region: IRb
length_bp: 485
variable_sites: 184
pi: 0.429
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-psbA_p1	GAAGCCCACCTCAAGATGAG	AACGCTCACAACTTCCCATT	55.0	45.0	58.0	58.0	877 bp	0.5	59.6
rrn5-psbA_p2	GAAGCCCACCTCAAGATGAG	TGCATCTTACATGGGTCGTG	55.0	50.0	58.0	58.0	1511 bp	0.5	59.5
rrn5-psbA_p3	ATCTCCCCCAAGAGTTCACA	AACGCTCACAACTTCCCATT	50.0	45.0	58.0	58.0	1182 bp	0.5	59.5
rrn5-psbA_p4	TCTCCCCCAAGAGTTCACAT	AACGCTCACAACTTCCCATT	50.0	45.0	58.0	58.0	1181 bp	0.5	59.5
rrn5-psbA_p5	TCTCCCCCAAGAGTTCACAT	TGCATCTTACATGGGTCGTG	50.0	50.0	58.0	58.0	1815 bp	0.5	59.5