Hotspot

trnK(ttt)-ycf12

LSC · 26502-26837 · 336 bp

Back to Guinardia

Pi 0.266

MarkerSeek score 80.20

Primer available yes

Label trnK(ttt)-ycf12

Feature Metadata

label_name: trnK(ttt)-ycf12
feature_type: igs
region: LSC
length_bp: 336
variable_sites: 86
pi: 0.266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK(ttt)-ycf12_p1	TCGAACCCAGAACAAATCGG	AGCTGACCTAAAACTTGCCA	50.0	45.0	58.2	57.3	422-429 bp	1.0	83.6
trnK(ttt)-ycf12_p2	GGACTCGAACCCAGAACAAA	AGCTGACCTAAAACTTGCCA	50.0	45.0	57.8	57.3	426-433 bp	1.0	83.2
trnK(ttt)-ycf12_p3	CGAACCCAGAACAAATCGGT	AGCTGACCTAAAACTTGCCA	50.0	45.0	58.5	57.3	421-428 bp	1.0	81.3
trnK(ttt)-ycf12_p4	TCGAACCCAGAACAAATCGG	ACCGGTTGCTACTAGCTGA	50.0	52.6	58.2	58.0	435-442 bp	1.0	80.6
trnK(ttt)-ycf12_p5	TCGAACCCAGAACAAATCGG	CACCGGTTGCTACTAGCTG	50.0	57.9	58.2	57.9	436-443 bp	1.0	80.4