Hotspot

trnM(cat)-trnR(ccg)

LSC · 20905-21248 · 344 bp

Back to Guinardia

Pi 0.123

MarkerSeek score 80.50

Primer available yes

Label trnM(cat)-trnR(ccg)

Feature Metadata

label_name: trnM(cat)-trnR(ccg)
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 41
pi: 0.123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM(cat)-trnR(ccg)_p1	ATTATGAGTCCGGTGCCTTC	GGTTCGATTCCCTTCATGCT	50.0	50.0	57.4	58.0	406-424 bp	1.0	84.9
trnM(cat)-trnR(ccg)_p2	GATTATGAGTCCGGTGCCTT	GGTTCGATTCCCTTCATGCT	50.0	50.0	57.4	58.0	407-425 bp	1.0	84.9
trnM(cat)-trnR(ccg)_p3	TATGAGTCCGGTGCCTTCAA	GGTTCGATTCCCTTCATGCT	50.0	50.0	58.7	58.0	404-422 bp	1.0	84.1
trnM(cat)-trnR(ccg)_p4	TTATGAGTCCGGTGCCTTCA	GGTTCGATTCCCTTCATGCT	50.0	50.0	58.7	58.0	405-423 bp	1.0	84.1
trnM(cat)-trnR(ccg)_p5	ATTATGAGTCCGGTGCCTTC	GGGGTTCGATTCCCTTCATG	50.0	55.0	57.4	58.3	408-426 bp	1.0	82.6