Hotspot

psaC

SSC · 123911-124156 · 246 bp

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Pi 0.563

MarkerSeek score 75.70

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 134
pi: 0.563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	ACCGGCTATTGTTTCGTCAA	AACCGATACCGAGAACTCCT	45.0	50.0	57.8	57.9	1270 bp	0.5	57.5
psaC_p2	GCTATTGGACCGGCTATTGT	AACCGATACCGAGAACTCCT	50.0	50.0	57.7	57.9	1278 bp	0.5	56.8
psaC_p3	GACCGGCTATTGTTTCGTCA	AACCGATACCGAGAACTCCT	50.0	50.0	58.3	57.9	1271 bp	0.5	56.6
psaC_p4	GGATTGATCACGAGCCGAAA	AACCGATACCGAGAACTCCT	50.0	50.0	58.3	57.9	1443 bp	0.5	56.1
psaC_p5	ACCGGCTATTGTTTCGTCAA	AAGAAACCCGCGGATATTGG	45.0	50.0	57.8	58.3	770 bp	0.5	56.0