Hotspot

ndhI

SSC · 121721-122206 · 486 bp

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Pi 0.463

MarkerSeek score 79.50

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 486
variable_sites: 200
pi: 0.463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	ACAGTTCTTTACTTGGGCGG	GTGCTGCAGCTACAAAATGG	50.0	50.0	58.1	58.0	1396 bp	0.5	59.0
ndhI_p2	CAGTTCTTTACTTGGGCGGT	GTGCTGCAGCTACAAAATGG	50.0	50.0	58.1	58.0	1395 bp	0.5	59.0
ndhI_p3	ACAGTTCTTTACTTGGGCGG	CAAGAACCAGTCCCAACGAA	50.0	50.0	58.1	58.0	1342 bp	0.5	58.8
ndhI_p4	CAGTTCTTTACTTGGGCGGT	CAAGAACCAGTCCCAACGAA	50.0	50.0	58.1	58.0	1341 bp	0.5	58.8
ndhI_p5	CAGTTCTTTACTTGGGCGGT	TCCCAACGAAAAGGCAGAAA	50.0	45.0	58.1	57.9	1331 bp	0.5	58.7