Hotspot

ndhH

SSC · 118132-119313 · 1182 bp

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Pi 0.464

MarkerSeek score 83.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 514
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GCAGCCGTTTCAGTATGGTA	CCGTCTGCTAGAGCTTGAAG	50.0	55.0	58.0	58.1	2069 bp	0.5	59.2
ndhH_p2	GCAGCCGTTTCAGTATGGTA	TTTGTTCCGTCTGCTAGAGC	50.0	50.0	58.0	57.9	2075 bp	0.5	59.2
ndhH_p3	GCAGCCGTTTCAGTATGGTA	TCAGGTCCAATACGCTGTTG	50.0	50.0	58.0	57.9	2027 bp	0.5	59.1
ndhH_p4	GAAAACCCGGGATCGATTGA	CCGTCTGCTAGAGCTTGAAG	50.0	55.0	58.0	58.1	1747 bp	0.5	59.1
ndhH_p5	GAAAACCCGGGATCGATTGA	TTTGTTCCGTCTGCTAGAGC	50.0	50.0	58.0	57.9	1753 bp	0.5	59.1