Hotspot

ndhG

SSC · 122575-123105 · 531 bp

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Pi 0.489

MarkerSeek score 80.30

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 230
pi: 0.489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	AATCAAATTGCTTTGGGCCG	TTGACGAAACAATAGCCGGT	45.0	45.0	57.9	57.8	1459 bp	0.5	57.8
ndhG_p2	AATCAAATTGCTTTGGGCCG	ACAATAGCCGGTCCAATAGC	45.0	50.0	57.9	57.7	1451 bp	0.5	57.2
ndhG_p3	AATCAAATTGCTTTGGGCCG	TGACGAAACAATAGCCGGTC	45.0	50.0	57.9	58.3	1458 bp	0.5	57.0
ndhG_p4	AATCAAATTGCTTTGGGCCG	TTTCGGCTCGTGATCAATCC	45.0	50.0	57.9	58.3	1286 bp	0.5	56.4
ndhG_p5	TTTGGGCCGTTTACCAATGT	TTGACGAAACAATAGCCGGT	45.0	45.0	58.3	57.8	1448 bp	0.5	56.3