Hotspot

ndhF

SSC · 128525-130813 · 2289 bp

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Pi 0.447

MarkerSeek score 79.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2289
variable_sites: 869
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TCCTGCTTACTTGGTCGTTT	GTTTCGATGCAACAACCAACT	45.0	42.9	57.1	57.9	2950 bp	0.5	44.0
ndhF_p2	TCCTGCTTACTTGGTCGTTT	TGTTTCGATGCAACAACCAAC	45.0	42.9	57.1	58.2	2951 bp	0.5	43.0
ndhF_p3	TCCTGCTTACTTGGTCGTTT	TGTTTCGATGCAACAACCAA	45.0	40.0	57.1	56.8	2951 bp	0.5	42.7
ndhF_p4	ATCCTGCTTACTTGGTCGTT	GTTTCGATGCAACAACCAACT	45.0	42.9	56.8	57.9	2951 bp	0.5	42.2
ndhF_p5	ATCCTGCTTACTTGGTCGTT	TGTTTCGATGCAACAACCAAC	45.0	42.9	56.8	58.2	2952 bp	0.5	41.2