Hotspot

ndhE

SSC · 123341-123646 · 306 bp

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Pi 0.482

MarkerSeek score 76.70

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 136
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	CCATTTTGTAGCTGCAGCAC	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	58.0	58.0	1312 bp	0.5	59.8
ndhE_p2	CCATTTTGTAGCTGCAGCAC	ACCCATGCTTCGAGTTGTTT	50.0	45.0	58.0	58.0	1406 bp	0.5	59.7
ndhE_p3	CCATTTTGTAGCTGCAGCAC	GCTTCGAGTTGTTTCATGCC	50.0	50.0	58.0	58.0	1400 bp	0.5	59.6
ndhE_p4	TTCGTTGGGACTGGTTCTTG	TCCTCTGTTCTTGGAGCAGA	50.0	50.0	58.0	58.0	1366 bp	0.5	59.6
ndhE_p5	TTCGTTGGGACTGGTTCTTG	ACCCATGCTTCGAGTTGTTT	50.0	45.0	58.0	58.0	1460 bp	0.5	59.5