Hotspot

ndhD

SSC · 124282-125778 · 1497 bp

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Pi 0.482

MarkerSeek score 82.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1497
variable_sites: 659
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TCTGCTCCAAGAACAGAGGA	TCAGGAGCAGTATGGGCTAA	50.0	50.0	58.0	57.8	2286 bp	0.5	58.5
ndhD_p2	TCTGCTCCAAGAACAGAGGA	TTCAGGAGCAGTATGGGCTA	50.0	50.0	58.0	57.8	2287 bp	0.5	58.5
ndhD_p3	AAACAACTCGAAGCATGGGT	TCAGGAGCAGTATGGGCTAA	45.0	50.0	58.0	57.8	2192 bp	0.5	58.5
ndhD_p4	AAACAACTCGAAGCATGGGT	TTCAGGAGCAGTATGGGCTA	45.0	50.0	58.0	57.8	2193 bp	0.5	58.5
ndhD_p5	GGCATGAAACAACTCGAAGC	TCAGGAGCAGTATGGGCTAA	50.0	50.0	58.0	57.8	2198 bp	0.5	58.4