Hotspot

ccsA

SSC · 126071-127042 · 972 bp

Back to Guilandina

Pi 0.444

MarkerSeek score 78.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 333
pi: 0.444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGTGTTTGTGAGAACCCCTT	GGGATATTGGGCAGCATTGA	50.0	50.0	57.9	58.0	1770 bp	0.5	59.5
ccsA_p2	GGTGTTTGTGAGAACCCCTT	AGGGATATTGGGCAGCATTG	50.0	50.0	57.9	58.0	1771 bp	0.5	59.4
ccsA_p3	GGTGTTTGTGAGAACCCCTT	GTGAAATCGGTAGACACGCT	50.0	50.0	57.9	58.0	1288 bp	0.5	59.4
ccsA_p4	GGTGTTTGTGAGAACCCCTT	TGAAATCGGTAGACACGCTG	50.0	50.0	57.9	58.0	1287 bp	0.5	59.4
ccsA_p5	CAGTTGACAAGGTCGAAGCT	GGGATATTGGGCAGCATTGA	50.0	50.0	58.1	58.0	1961 bp	0.5	59.0