Hotspot

rpl32-ndhF

SSC · 127767-128524 · 758 bp

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Pi 0.409

MarkerSeek score 81.00

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 758
variable_sites: 266
pi: 0.409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TCAATGCTGCCCAATATCCC	TAGGGCGTGTGAACCAAAAA	50.0	45.0	58.0	57.9	1354 bp	0.5	59.6
rpl32-ndhF_p2	CAATGCTGCCCAATATCCCT	TAGGGCGTGTGAACCAAAAA	50.0	45.0	58.0	57.9	1353 bp	0.5	59.5
rpl32-ndhF_p3	AGCGTGTCTACCGATTTCAC	TAGGGCGTGTGAACCAAAAA	50.0	45.0	58.0	57.9	1836 bp	0.5	59.5
rpl32-ndhF_p4	CAGCGTGTCTACCGATTTCA	TAGGGCGTGTGAACCAAAAA	50.0	45.0	58.0	57.9	1837 bp	0.5	59.5
rpl32-ndhF_p5	TCAATGCTGCCCAATATCCC	AGCAACTGAACCGCCAAATA	50.0	45.0	58.0	58.1	1563 bp	0.5	59.2