Hotspot

ndhA-ndhI

SSC · 121642-121720 · 79 bp

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Pi 0.48

MarkerSeek score 77.30

Primer available yes

Label ndhA-ndhI

Feature Metadata

label_name: ndhA-ndhI
feature_type: igs
region: SSC
length_bp: 79
variable_sites: 36
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhI_p1	ACAGTTCTTTACTTGGGCGG	CGGCCCAAAGCAATTTGATT	50.0	45.0	58.1	57.9	789 bp	0.5	58.4
ndhA-ndhI_p2	CAGTTCTTTACTTGGGCGGT	CGGCCCAAAGCAATTTGATT	50.0	45.0	58.1	57.9	788 bp	0.5	58.4
ndhA-ndhI_p3	ATTTATGGCCTTCGAGGAGC	CGGCCCAAAGCAATTTGATT	50.0	45.0	57.7	57.9	1141 bp	0.5	57.1
ndhA-ndhI_p4	TTTACTTGGGCGGTTGGAAT	CGGCCCAAAGCAATTTGATT	45.0	45.0	57.7	57.9	782 bp	0.5	56.8
ndhA-ndhI_p5	CAGTTCTTTACTTGGGCGGT	ACATTGGTAAACGGCCCAAA	50.0	45.0	58.1	58.3	799 bp	0.5	56.8