Hotspot

trnH-GUG-psbA

LSC · 79-393 · 315 bp

Back to Guilandina

Pi 0.078
MarkerSeek score 88.30
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
315
variable_sites
23
pi
0.078
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
88.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 384-390 bp 1.0 87.1
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 398-404 bp 1.0 86.9
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 397-403 bp 1.0 86.9
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 399-405 bp 1.0 86.9
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCGAGCTAACCTTGGTATGG 55.0 55.0 58.1 57.8 433-439 bp 1.0 84.9