Hotspot

ycf1

SSC · 127618-133260 · 5643 bp

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Pi 0

MarkerSeek score 52.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5643
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGCCATTAGATCCGTGTCAT	CTTGCCAGAATTTGTGCCAC	50.0	50.0	57.8	58.2	1197 bp	1.0	77.1
ycf1_p2	CGCCATTAGATCCGTGTCAT	TCTTGCCAGAATTTGTGCCA	50.0	45.0	57.8	58.3	1198 bp	1.0	76.4
ycf1_p3	CGCCATTAGATCCGTGTCAT	CCGGTTCTTGCCAGAATTTG	50.0	50.0	57.8	57.6	1203 bp	1.0	75.9
ycf1_p4	CGCCATTAGATCCGTGTCAT	GCCAGAATTTGTGCCACTTT	50.0	45.0	57.8	57.5	1194 bp	1.0	74.5
ycf1_p5	CGCCATTAGATCCGTGTCAT	TGCCAGAATTTGTGCCACTT	50.0	45.0	57.8	58.6	1195 bp	1.0	74.1