Hotspot

psbA

LSC · 210-1271 · 1062 bp

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Pi 0.00283

MarkerSeek score 49.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GGAGAGCCCCAATTGATACA	GTGTGCTTGGGAGTTCTTGA	50.0	50.0	57.0	58.0	1141 bp	0.5	51.5
psbA_p2	GGAGAGCCCCAATTGATACA	ATACGTGTGCTTGGGAGTTC	50.0	50.0	57.0	57.9	1145 bp	0.5	51.0
psbA_p3	GGAGAGCCCCAATTGATACAC	GTGTGCTTGGGAGTTCTTGA	52.4	50.0	58.4	58.0	1141 bp	0.5	48.4
psbA_p4	GGAGAGCCCCAATTGATACAC	ATACGTGTGCTTGGGAGTTC	52.4	50.0	58.4	57.9	1145 bp	0.5	47.9
psbA_p5	GGAGAGCCCCAATTGATACA	AATACGTGTGCTTGGGAGTT	50.0	45.0	57.0	57.4	1146 bp	0.5	47.3