Hotspot

atpF

LSC · 11847-13196 · 1350 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1350
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCCT	45.0	37.5	57.4	57.5	1504 bp	1.0	40.0
atpF_p2	ACTAATTTCGTCGGCTCGAA	AGTCCTATCTATAAGAGGAGAGCA	45.0	41.7	57.4	57.3	1486 bp	1.0	40.0
atpF_p3	GGCTCGAAGGGTTACCATTA	AGAACTCTGTTCTTTGTTAGTCCT	50.0	37.5	57.0	57.5	1492 bp	1.0	40.0
atpF_p4	ACTAATTTCGTCGGCTCGAA	AGAACTCTGTTCTTTGTTAGTCC	45.0	39.1	57.4	56.2	1504 bp	1.0	40.0
atpF_p5	ACTAATTTCGTCGGCTCGAA	GAACTCTGTTCTTTGTTAGTCCT	45.0	39.1	57.4	56.2	1503 bp	1.0	40.0